Enzymatic acylation of flavone isolated from extractive of bamboo leaves with oleic acid and antioxidant activity of acylated product.

Abstract

This present study aims to establish a relatively cheap and effective method for preparation of ester of flavone isolated from bamboo-leaf extracts. According to this method, the solubility of bamboo leaf extract in a lipid medium could be improved, which would expand the utilization of bamboo leaf extract. Acylation of flavone with oleic acid was efficient in a system containing dried tert-amyl-alcohol and molecular sieves. The bioconversion yield ranged from 24 to 63% in different conditions. the type of fatty acids, the amount of the lipase, water content and temperature of the system were investigated in detail to influence on the performance of the acylation reaction. High purity of ester (>95%) was obtained by a two-step solvent purification procedure and chromatographic separation. Followed by the 1H and 13C nuclear magnetic resonance spectroscopy analysis, single product of 6''-O-oleate isoorientin ester was detected. Introducing an acyl group into isoorientin significantly improved its lipophilicity yet slightly reduced their antioxidant activity, while its antioxidant activity still significantly better than VE.