Abstract
Introduction:Microorganisms are a preferred source of enzyme production due to their high production capability and low cost of production. Bacterial endophytes occupy unexplored sites hence they represent a new source of enzymes with diverse applications. Mangrove plants in Kenya have traditionally been used as medicinal plants due to their bioactive metabolites. However the enzymatic activity of mangrove plants associated endophytes has not been studied.Aims & Objectives:The study is aimed at bioprospecting for enzymes with potential biotechnological applications from mangrove ecosystems.Methods & Materials:Forty-two bacterial isolates were cultured and isolated from the leaves and roots of six mangrove plants sampled from Mida Creek and Gazi Bay in the coastal region of Kenya. The isolates were screened for pectinases, chitinases, cellulases, proteases, and amylases. The isolates were identified based on morphology and 16S rRNA gene sequences analysis.Results:The study showed bacterial isolates had enzymatic activity as follows; pectinases activity (69% of the isolates), Proteases (95% of the isolates), amylases activity (88% of the isolates), cellulases and chitinases (92% of the isolates each). Bacterial endophytes from leaves showed a higher enzymatic index of cellulases suggesting a potential role in degrading cellulose in the leaves of plants. The enzymes amylases and proteases were mostly exhibited by endophytes in roots suggesting a potential role in metabolizing sugar and amino acids in the roots. Isolates from the mangrove plantSonneratia albashowed highest enzymatic indices. The study also observed that isolates from mangrove plants sampled from Gazi bay had high means of enzymatic indices. Molecular identification showed the isolates were closely related toBacillus, Streptomyces, Myroides, andStaphylococcusspecies. Their respective enzymatic activities have been provided in this study.Conclusion:The study showed that Kenyan Mangrove plant-associated bacterial endophytes provide a good reservoir of enzymes with potential industrial applications.
Highlights
Microorganisms are a preferred source of enzyme production due to their high production capability and low cost of production
The study showed that Kenyan Mangrove plant-associated bacterial endophytes provide a good reservoir of enzymes with potential industrial applications
The study revealed that Mangrove plants from Kenya’s coastal region harbor endophytes with potential enzymes that can be applicable in industries such as detergent, textile, and agrochemical
Summary
Microorganisms are a preferred source of enzyme production due to their high production capability and low cost of production. Plant-associated endophytes are a potential source of enzymes such as cellulases that can be used in biotechnological and pharmaceutical processes [6]. These hydrolytic enzymes can be used in bioremediation process since they can break down complex biopolymers into sugars [7]. Endophytes in mangrove plants occupy a relatively unexplored site in microorganism isolation and can be a source for enzymes that can be utilized in food processing, detergent industries and pharmaceutical companies [9]. The intimate and non-detrimental association between endophyte, their host plant and the complex substrates involved may provide a unique habitat causing the endophytes to develop various mechanisms producing a variety of bioactive compounds [10] or bioprospecting for potential industrial enzymes. The objectives of the study were to isolate, identify and enzymes screening of bacterial endophytes from leaves and roots of selected mangrove plants found in at Mida Creek and Gazi Bay in Kenya coastal region
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