Abstract
The present research was aimed to study enzymatic activity (cellulase and chitosanase) and secondary metabolite production of Trichoderma asperellum in presence of chitosan. The qualitative assays for cellulase and chitosanase showed enhanced enzymatic activity of the fungus. The H/C ratio obtained was 1.76 and 1.49 at 500 ppm and 250 ppm of chitosan respectively, which was significantly higher, as compared to control (H/C ratio 1.2). For chitosanase activity, the largest purple zone (55.8 mm) was recorded in case of T. asperellum + chitosan 500 ppm which was significantly higher than other treatments. Besides enhancing enzymatic activity, chitosan also increased the production of two important secondary metabolites of T. asperellum viz. Tetradecanoic acid (myristic acid) and 1,2-benzenedicarboxylic acid. GC–MS results revealed that the maximum peak area of chromatogram was covered by tetradecanoic acid (28.23%) followed by 1,2-benzenedicarboxylic acid (18.16%) showing the high concentration of these metabolites as compared to other compounds. The enhanced production of cellulase, chitosanase and secondary metabolites by T. asperellum in presence of chitosan could be exploited for further research in improvement of bio-control activity of bio-agents for integrated plant disease management.
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