Abstract
The volume and diversity of biological data are increasing at very high rates. Vast amounts of protein sequences and structures, protein and genetic interactions and phenotype studies have been produced. The majority of data generated by high-throughput devices is automatically annotated because manually annotating them is not possible. Thus, efficient and precise automatic annotation methods are required to ensure the quality and reliability of both the biological data and associated annotations. We proposed ENZYMatic Annotation Predictor (ENZYMAP), a technique to characterize and predict EC number changes based on annotations from UniProt/Swiss-Prot using a supervised learning approach. We evaluated ENZYMAP experimentally, using test data sets from both UniProt/Swiss-Prot and UniProt/TrEMBL, and showed that predicting EC changes using selected types of annotation is possible. Finally, we compared ENZYMAP and DETECT with respect to their predictions and checked both against the UniProt/Swiss-Prot annotations. ENZYMAP was shown to be more accurate than DETECT, coming closer to the actual changes in UniProt/Swiss-Prot. Our proposal is intended to be an automatic complementary method (that can be used together with other techniques like the ones based on protein sequence and structure) that helps to improve the quality and reliability of enzyme annotations over time, suggesting possible corrections, anticipating annotation changes and propagating the implicit knowledge for the whole dataset.
Highlights
In recent decades there has been a surge in the amount of biological data available
Descriptive Multiclass Experiment we present the results of the descriptive step. This experiment aimed to verify whether the line types Organism Classification (OC), Reference Position (RP) and KW are able to discriminate entries that experienced a specific change in their EC number from those that remained the same
We proposed ENZYMatic Annotation Predictor (ENZYMAP), a technique based on supervised learning to characterize and predict annotation changes in temporal data from UniProt/Swiss-Prot using entry line types that are already available in the database
Summary
In recent decades there has been a surge in the amount of biological data available. According to [1], new DNA sequencing technologies allowed a 1000-fold drop in sequencing costs since 1990 and made an increasing number of large data collection projects economically possible, leading to an exponential increase in the DNA sequence data available. Glycoprotein G of the Nipah virus (entry Q9IH62 in Swiss-Prot) illustrates the drawbacks of this approach. Up to release 14 (July 2008) of UniProt/Swiss-Prot [9], entry Q9IH62 was considered an enzyme. Despite all these sequence and structural similarities, Henipavirus Glycoproteins G are known not to be enzymes and to have only hemagglutinin activity, performing protein-protein interactions with host receptors [7]. The scientific community still has concerns regarding the quality and reliability of the data and annotations from the large, publicly available databases
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
