Abstract
In this study, the green extraction of Bioactive compounds and antioxidant activity from Rosehip (Rosa canina L.) fruits was investigated using ultrasound-assisted extraction combined with deep eutectic solvents (DES). A deep eutectic solvent-based on citric acid has been specially designed. Hydrogen bond donors (HBD) such as glycerol and ethylene glycol were used and a hydrogen bond acceptor (HBA) like citric acid was used. After choosing the best option of DES, extraction of the bioactive ingredients, optimal extraction conditions of the ultrasonic-assisted extraction have been optimized through the Box-Behnken design of response surface methodology (RSM). Total phenolics content (TPC), total anthocyanins content (TAA), and antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) have been found as 103.37 mg GAE/g DW in DES2, 92.23 mg GAE/g DW in DES1, 3.25mg C3G/100g-DW in DES2,1.31 mg C3G/100g-DW in DES1, and 101.85% Inhibition in DES2, 94.32 %. The results showed that this method is a competitive sustainable, green, and effective extraction of bioactive compounds from Rosehip (Rosa canina L.) fruits.
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