Abstract
The symbiotic system of Legumes and root-nodule bacteria is a significant process for enriching barren and dry soils and supporting plant growth. The aim of this study was to isolate root-nodule bacteria from Medicago falcate and to select high activity strains with resistance to environmental stress. As a result, only MSZ-25 strain grew in the medium containing 0.8M NaCl, with pH4.8-9.8 and at temperatures of 4-45°С. All rhizobial isolates were tolerant to the concentrations of 0.01-0.1% Pb(NO3)2, 0.01% ZnSO4, CdCI2, CuCI2 and NiCI2. The strains were also resisted to penicillin 10μ/ml, erythromycin 10μ/ml, and chlorampenicol 10μ/ml but the growths of all isolates were inhibited by kanamycin 30μ/ml, novobiocin 30μ/ml and tetracycline 10μ/ml.
Highlights
select high activity strains with resistance to environmental stress
only MSZ-25 strain grew in the medium containing
at temperatures of 4-45°С. All rhizobial isolates were tolerant to the concentrations of 0.01-0.1% Pb(NO3)2
Summary
ЦАРГАСНЫ (MEDICAGO FALCATE L) ҮНДЭСНИЙ БУЛЦУУНЫ БАКТЕРИЙН ГАДААД ОРЧНЫ ХҮЧИН ЗҮЙЛС ТЭСВЭРЛЭХ ЧАДВАР. Буурцагт ургамал (Fabaceae)- булцууны бактеритай (Rhizobium) ашигтай симбиоз үүсгэдэг систем нь хөрсний үржил шимийг сайжруулах, ургамлан бүрхэвчийг нөхөн сэргээхэд чухал ач холбогдолтой юм. Энэхүү судалгаагаар буурцагт ургамал - царгас (Medicago falcate L)-аас булцууны бактерийн өсгөврүүдийг ялгаж, тэдгээрээс гадаад орчны хүчин зүйлст тэсвэрлэх өндөр идэвхтэй өсгөвөр илрүүлэхийг зорилоо. Түлхүүр үгс: Medicago falcate L; Хянгар царгас; булцууны бактери; гадаад орчны хүчин зүйлс; ОРШИЛ. Буурцагт ургамал (Fabaceae) нь мал аж ахуйд голлох тэжээлийн ургамал болохоос гадна хуурай давстай газар нутгийн доройтсон хөрсний шимийг сайжруулах, мөн ургамлан бүрхэвчийг нөхөн сэргээх зэргээр экосистемийн тэнцвэрт байдлыг хадгалан үлдэхэд түүний булцууны бактеритай (Rhizobium) үүсгэдэг симбиоз систем чухал ач холбогдолтой [7, 11, 12].
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