Environmental selection of marine stramenopile clades in the Arctic Ocean and coastal waters

Abstract

Single-celled bacterial grazers are key components of marine ecosystems, and at times represent the dominant eukaryotes in the Arctic Ocean. Among these are small, phylogenetically diverse stramenopiles, known almost exclusively from their 18S rRNA gene sequences. Marine stramenopiles (MAST) have been found in the upper waters of the world ocean and in all Arctic seas. In particular, three sub-clades of MAST-1, MAST-1A, 1B and 1C co-occur in Arctic waters, but ecological and distributional details are lacking. Here, we have updated phylogenies of the MAST-1 clade to test whether there are Arctic-specific MAST-1 ecotypes. In addition, taking advantage of samples collected over 2 years as part of several Arctic cruises leading up to and during the International Polar Year, we applied sub-clade-specific probes and fluorescent in situ hybridization to describe the distribution of the three sub-clades in the Canadian Arctic. The three groups were found principally in the euphotic zone; however, evidence of Arctic ecotypes remains elusive. Some environment-specific separation among sub-clades was detected, with MAST-1C reaching significantly greater concentrations near the marginal ice zone, while MAST-1A and MAST-1B were associated with ice-covered and open-water stations, respectively. MAST-1B appeared to be able to persist at greater depths. Changing ice cover and mixing regimes are therefore expected to have an impact on the concentrations and distribution of these sub-clades, with possible downstream consequences on the marine food webs.