Environmental forensics evaluation of residual soybean sludge using trees of Brazilian savannah

Abstract

Truck and train are the primary transport means for soybean shipments to national or international markets from Brazil. Substantial amounts of grain residues are dispersed throughout the transport and may accumulate in various places by the runoff. The objective of this study was to evaluate the contamination and mortality of Brazilian savannah trees due to the accumulation of soybean runoff that comes from a grain storage yard. Xylem samples were collected from 28 dead trees in a forest where residues severely polluted the natural environment. Increment cores at breast height were also taken from 25 living trees for control procedure. All samples were analyzed by X-ray densitometry and microfluorescence (XRF) techniques. No differences were observed in the xylem densitometry profile from bark to pith between dead and living trees, indicating that the stagnation in cell production and tree growth occurred drastically after the accumulation of soybean residual sludge, which was confirmed by temporal analysis of satellite images. Significantly higher S, P, Ca and Fe concentrations were observed close to the bark in dead trees. The roots of dead trees accumulated higher amounts of Al, Si, S, Ca and Mn. Higher K, Ca and Si concentrations and lower Mn concentrations were observed in trees closer to the polluted area, and it indicates the need for soil remediation. However, As, Pb and Cd, markers of fertilizers and pesticides in soybean production, were not detected by the analyses performed. It is likely that the residual soybean sludge and its fermentation process during the rainy season (tree - growing season) have generated toxic concentrations of inorganic constituents, that together with warmer soil conditions caused the death of many trees. This study provides tools for the assessment of the environmental impact of soybean production through a novel protocol for monitoring the physical and chemical patterns of tree growth using rapid microscopic scale X-ray techniques.