Environmental drivers on seasonal abundance of riverine-estuarine V. cholerae in the Indian Sundarban mangrove

Abstract

Gangetic delta is considered as the homeland of cholera, which is thought to be influenced by changes in populations of estuarine Vibrio cholerae. We aim to identify the environmental, biotic and abiotic driving forces influencing the V. cholerae dynamics in riverine-estuarine environment of southern deltaic Bengal. Cultivable Vibrio count (CVC) ranged between 1 and 103 colony forming units (CFU)/mL at a salinity gradient of 1.9–30 practical salinity unit (PSU). Increased water temperatrure during summer influences the higher CVC followed by a sudden fall along with the onset of monsoon upto winter. While summer V. cholerae O1 peak (50–100CFU/mL) can be associated with higher water temperature (P<0.05) and higher turbidity (P<0.005); sharp fall during monsoon (15–45CFU/mL) is attributed to reduced salinity (25–2.5PSU). Plankton attached V. cholerae O1 varied between 10 and 1000CFU/mL with a highest peak at winter followed by summer and monsoon. Prevalence of toxigenic V. cholerae O1 in low salinity (2–7.5PSU) during monsoon identifies that high water temperature (>25°C), higher turbidity (>100NTU) and lower salinity plays the pivotal role in toxicity acquisition. Present investigation establishes the role of Sundarban mangrove, where V. cholerae exist in an avirulent condition. During migration towards low saline inland system, V. cholerae pool possibly acquires toxin genes under the influence of environmental factors. Planktonic attachment is possibly a survival strategy at adverse condition, when they do not acquire any toxin gene. Seasonal V. cholerae dynamics has been thoroughly established in environmental settings of high saline mangrove and brackish water flowing to inland low saline condition.