Abstract
Shewanella species are widespread in various environments. Here, the genome sequence of Shewanella piezotolerans WP3, a piezotolerant and psychrotolerant iron reducing bacterium from deep-sea sediment was determined with related functional analysis to study its environmental adaptation mechanisms. The genome of WP3 consists of 5,396,476 base pairs (bp) with 4,944 open reading frames (ORFs). It possesses numerous genes or gene clusters which help it to cope with extreme living conditions such as genes for two sets of flagellum systems, structural RNA modification, eicosapentaenoic acid (EPA) biosynthesis and osmolyte transport and synthesis. And WP3 contains 55 open reading frames encoding putative c-type cytochromes which are substantial to its wide environmental adaptation ability. The mtr-omc gene cluster involved in the insoluble metal reduction in the Shewanella genus was identified and compared. The two sets of flagellum systems were found to be differentially regulated under low temperature and high pressure; the lateral flagellum system was found essential for its motility and living at low temperature.
Highlights
The deep sea, which is characterized by extremely low temperatures (,5uC) and high pressures, comprises the bulk of the world’s oceans
The genome of WP3 is mostly related to S. loihica PV-4 genome, a psychrotolerant bacterium isolated from iron-rich microbial mats at an active, deep-sea, hydrothermal Naha vent (1,325 m below sea level) located on the South Rift of Loihi Seamount, Hawaii [21,22]
The deep-sea bacterium Photobacterium profundum strain SS9 has two sets of flagella genes [4] and the results presented here may extend to multiple genera of deep-sea microbes
Summary
The deep sea, which is characterized by extremely low temperatures (,5uC) and high pressures (up to 110 MPa), comprises the bulk of the world’s oceans. Genome sequence information of bacteria from high pressure and/or cold environments (including Photobacterium profundum [4], Colwellia psychrerythraea [5], Idiomarina loihiensis [6] and Pseudoalteromonas haloplanktis [7]) has indicated some of the relevant adaptation strategies. There are more than 18 Shewanella strains from various environments that have been sequenced by the Joint Genome Institute and other organizations [10,14]. These sequences provide a powerful frame of reference for additional genomic analyses of new Shewanella isolates possessing distinctive phenotypes
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
