Abstract
Using the extinction dilution method with tissue culture microplates, we examined conditions for the enumeration of viable bacteria that promote sperm formation in Coscinodiscus wailesii Gran (Bacillariophyceae) (BPSF-Cw; bacteria-promoting sperm formation in C. wailesii). Samples of bacteria were collected from the surface and bottom (1 m above the bottom) layers of seawater from Harima-Nada, eastern Seto Inland Sea, Japan, during August 1993 and from a sediment sample collected June 1994. The most probable numbers (MPN) of BPSF-Cw in the samples of seawater (surface and bottom) and the sediment were calculated to be 9.5 × 103 cells ml−1, 4.8 × 103 cells ml−1, and 6.3 × 104 cells g−1 wet sediment, respectively. This finding suggests that BPSF-Cw are abundant in the marine environment and that it should be possible to investigate the interaction between C. wailesii and BPSF-Cw in nature using this microplate MPN method.
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