Abstract
Jembrana disease (JD) caused by Jembrana Disease Virus (JDV) becomes an obstacle in Bali cattle (Bos javanicus). The development of JD vaccines has a critical meaning to prevent losses in the B. javanicus in Indonesia and is one of the models in the development of the Human Immunodeficiency Virus (HIV) vaccine. The development of vaccines for JDV has carried out DNA vaccines that are expected to provide better immunological effects. This study aimed to determine the low molecular weight chitosan (LC) entrapment towards pEGFP-C1-env-Tm in the formation of Chitosan Nanoparticles Low/pEGFP-C1-env-Tm complex. The env-Tm gene was inserted in pEGFP-C1 into the pEGFPC1/ env-Tm construct transformed on the E. coli DH5α host. The construct was formulated into LC/pEGFP-C1/env-Tm complex with a low molecular weight chitosan concentration of 0.06 % and the ratio of pEGFP-C1/env-Tm: LC (wt/wt) was 1:0.5-1:3. The complexes were then analyzed by gel retardation assay agarose 1 %. The results of this study indicated that the best entrapment results of low molecular weight chitosan to pEGFPC1/ env-Tm was in the mass ratio of pEGFP-C1/env-Tm: LC was 1:2. The best formulation entrapment for env-Tm by low molecular weight chitosan 0.06 % is in the rate 1:2.
Highlights
The obstacle faced by Bali cattle is Jembrana Disease (JD), which is caused by bovine lentivirus, a member of the family retroviridae, subfamily lentiviridae
Several different DNA mass ratios and low molecular weight chitosan were used, and only in the 1:2 and 1:3 ratio were complexes formed, seen with entrapped LC/pEGFP-C1/env-Tm complexes in the well
Low molecular weight chitosan solution as a negative control cannot bind DNA, and it can show the absence of the band
Summary
The obstacle faced by Bali cattle is Jembrana Disease (JD), which is caused by bovine lentivirus, a member of the family retroviridae, subfamily lentiviridae. This disease can affect the fertility of Bali cattle, thereby reducing the production of Bali cattle, which can affect economic conditions in Indonesia [1−5]. The delivery of pDNA using nonviral vectors results in gene expression, which encourages the development of new delivery systems that can protect pDNA, as well as localize its delivery, such as chitosan. This research was carried out on the use of low molecular weight chitosan as a protective molecule as well as carrying the target gene to cells
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