Entomopathogenicity ofSimplicillium lanosoniveumCG888 on Mortality ofCallosobruchus maculatusF. in vitro

Abstract

Research regardingSimplicillium lanosoniveumas an entomopathogen is still limited. The entomopathogenicity ofSimplicillium lanosoniveumCG888 with three different conidia density treatments, namely 1x106/ml, 1x107/ml, and 1x108/ml, was tested onCallosobruchus maculatusF. imago in the laboratory, incubated at 26-27°C, RH 70% and in the dark conditions. Based on the effect of conidia density,S. lanosoniveumCG888 at a density of 1x108conidia/ml caused greater mortality ofC. maculatusF, reaching 51.65% compared to other treatments and control. The influence of the length of time after inoculation was that there was 60.83%C. maculatusF. death on the 11thday. It was higher than the result on 3, 5, 7 and 9 days later. There was a positive interaction that the mortality ofC. maculatusF was influenced by the density ofS. lanosoniveumCG888 conidia and the period of days after inoculation. Among these treatments,S. lanosoniveumCG888 with 1x108/ml caused mortality of 86,7% inC. maculatuson day 11 after inoculation. The lowest mortality ofC. maculatusF. was in the density of 1x106conidia/ml of 70%. These findings indicate thatS. lanosoniveumCG888 can be used to controlC. maculatusF. safely and reduce the use of chemicals in soybean storage.