Abstract
In this study, the Nile River was investigated from Abu‐Simbol to Aswan for the presence of enteroviruses. The area of study includes Lake Nasser, the High Dam and the Aswan Dam (M 300 km). Forty litres of Nile River water was collected from each site and concentrated for virus detection and isolation. Plaque assay was used for virus counting in buffalo green monkey (BGM) cells followed by the neutralization test. Plaque purification was carried out to separate mixed types of viruses in each sample. Each purified plaque was reinoculated in BGM cells and viruses isolated were identified by dot‐ELISA to separate polioviruses. Enteroviruses were detected in nine sites especially at Abu‐Simbol region. The most common virus detected was poliovirus type 2 and the virus count ranged from 6 to 42 pfu/l. All polioviruses isolated were wild strains when tested against thermal stability.
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