Enteropancreatic Endocrine Cells Response to Streptozotocin Induced Hyperglycemia. (LB16)

Abstract

The Enteroendocrine cells form the largest endocrine system in the body. They secrete multiple regulatory molecules which control physiological and homeostatic functions particularly postprandial regulation of glucose and GIT motility (Sternini et al. 2008).In Diabetic setting, it is speculated that enteroendocrine cells which secrete hormones involved in carbohydrate metabolism will undergo changes aimed at maintaining carbohydrate homeostasis (Stephanie et al. 2009).To determine how enteroendocrine cells respond to diabetes and their likely role in diabetic complications, Sixty albino Wister rats (male=30 and female =30), weighing between 200‐300g were divided into five experimental groups (n=10) per group and a control (n=10) . Diabetes mellitus was induced with a single intraperitoneal dose of 65mg per kg of streptozotocin. Blood was sample from tail vein 30 minutes after the application of EMLAR cream (2.5% lignocaine and 2.5% prilocaine) for glucose test and only those with blood glucose within the ranges of 250‐ 600mg/dl were anaesthetized with isofluran , cervical dislocation was done to sacrifice the animals. (group 1= 2weeks, group 2=4weeks, group3=6weeks, group4=8weeks, group5=12 weeks). Experimental animals without selective beta cells destruction of the pancreas, demonstrated by chrome alum hematoxylin‐phloxine staining technique were excluded from the study. The stomach, duodenum, jejunum, ileum and large intestine were collected fixed in boin’s fluid and paraffin sections, 5 microns thick were stained using lead hematoxylin, Toluidine blue metachromasia, and the Grimelius methods. There was an increase in enteroendocrine cell population in the small intestine, large intestine; however few enteroendocrine cells were stained in the stomach when compared to matched control. Toluidine blue metachromatic staining technique stained enteroendocrine cell intensely in the jejunum and ileum when compared to matched control. These findings suggest a proliferation of enteroendocrine cell and by implication an increase in enteroendocrine cell hormones in chronic diabetes. Therefor it is reasonable to conclude that, enteroendocrine cells and their hormones are likely involved in the complications and management of diabetes mellitus.