Enterococcus mundtii isolated from cow dung and its probiotic, antioxidants and antigenotoxic potential

Abstract

The present study documents the potential probiotic LAB isolated from indigenous microflora of cow dung. In this study, isolated culture was analyzed using 16S ribosomal RNA gene sequencing leading to the identification of LAB isolates (Enterococcus mundtii). The isolate was found to be non-hemolytic and can tolerate different bile, pH and NaCl and showed growth at 45° C. The culture survived gastric and intestinal environments and showed bile salt hydrolysis activity. Isolate exhibited autoaggregation (96%) and hydrophobic reaction (94%). Antimicrobial activities of cell-free supernatant (CFS) of isolates were estimated through standard agar-well diffusion assay against commonly occurring food-borne and clinically important human pathogens. CFS of the isolate showed maximum inhibitory activity against pathogen. Antigenotoxic (comet assay) and antioxidant (DPPH) potential were also performed for the selected isolate. Therefore, this strain should be subjected to the other required tests to prove its suitability for clinical therapeutic application. The selected isolate showed the highest activity as antimicrobial, antigenotoxic and antioxidant.