Abstract
Recent studies have demonstrated the immunomodulatory effects of heat-killed lactic acid bacteria. The aim of this study was to evaluate the protective effect of heat-killed Enterococcus faecalis EF-2001 (EF-2001) on a model of inflammatory bowel disease (IBD). A total of 28 female NC/Nga mice were divided into 4 treatment groups. Controls were fed a normal commercial diet. In the experimental groups, colitis was induced by rectal administration of dinitrobenzene sulfonic acid. Two groups were orally administered 2 and 17 mg/kg EF-2001, respectively. EF-2001 treatment decreased the expression of several cytokines, including cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), interferon (IFN)-γ, interleukin (IL)-1β, and IL-6 in inflamed colon compared to the DNBS alone group. In addition, EF-2001 suppressed DNBS-induced colonic tissue destruction. Therefore, this study strongly suggests that EF-2001 could alleviate the inflammation associated with mouse IBD.
Highlights
Several probiotic bacteria have been reported as favorable candidates for the treatment and prevention of disease through the regulation of the host immune system [1,2]
In the disease activity index, we found the dinitrobenzene sulfonic acid (DNBS)+EF-2001 1TM and DNBS+EF2001 groups disease activity scorings were lower than DNCB group (Fig 1E)
We observed the increased-mesenteric lymph nodes in the DNBS group compared with the control group, and EF-2001 treatment reduced mesenteric lymph node weight compared to the DNBS group (Fig 2D and 2E)
Summary
Several probiotic bacteria have been reported as favorable candidates for the treatment and prevention of disease through the regulation of the host immune system [1,2]. Consuming probiotic foods, such as yogurt, has been reported to improve abnormal immune function [3,4]. In addition to live lactic acid bacteria, heat-killed cells display immunomodulatory functions [5,6]. Inflammatory bowel disease (IBD) is a group of inflammatory disorders of the digestive tract that includes Crohn’s disease (CD) and ulcerative colitis (UC).
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