Enterococcus durans EP1 modulates inflammation in a DSS-induced colitis model in mice

Abstract

Event Abstract Back to Event Enterococcus durans EP1 modulates inflammation in a DSS-induced colitis model in mice Paula Carasi1, 2*, Silvia Racedo2, Claudine Jacquot2, María Serradell1 and María C. Urdaci2 1 Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Dep. de Ciencias Biológicas, Argentina 2 Universite de Bordeaux, Bordeaux Science Agro, France Enterococcus durans EP1, a gram-positive coccoid bacteria, has shown various probiotic properties. The objective of this work was to evaluate the immunomodulatory capacity of EP1 in a colitis model induced by dextran sulfate sodium (DSS) in mice. Six weeks old Swiss mice were separated in two groups of 16 animals. During 14 days, one group received daily 108 cfu/mice of EP1 and the other received PBS. On day 14, each of the mentioned groups was separated in two (eight mice each) and administration of DSS 3% w/v started for one of them and lasted seven days. Significant differences in the disease activity index were observed between DSS group and DSS treated with EP1 mice (DSS-EP1), DSS mice´s colons were shorter (p<0.05) than the colons from control and DSS-EP1 mice. Histological studies were consistent with these findings. Cytokines production by colon explants ex vivo showed that DSS mice secreted higher levels of IL6, IL17A, IFNγ, TNFα and GM-CSF compared with control and DSS-EP1 mice but no differences were observed for IL10. Gene expression studies demonstrated that in colon the expression of IL17A, INFγ, IL1β and CXCL1 was lower in DSS-EP1 group compared with DSS mice. In mLN, expression of IL23, IL17A, RORγT and GM-CSF was significantly decreased in DSS-EP1 group. Moreover, EP1 treatment partially recomposed the changes in the microbiota induced by DSS. These results indicate that administration of E. durans EP1 attenuates damages generated by DSS by decreasing expression and secretion of proinflammatory mediators, as well as modulating microbiota. Keywords: DSS, Probiotics, intestinal inflammation, Enterococcus, microbiota Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Carasi P, Racedo S, Jacquot C, Serradell M and Urdaci MC (2013). Enterococcus durans EP1 modulates inflammation in a DSS-induced colitis model in mice. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00432 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Paula Carasi, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Dep. de Ciencias Biológicas, La Plata, Argentina, paulacarasi@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Paula Carasi Silvia Racedo Claudine Jacquot María Serradell María C Urdaci Google Paula Carasi Silvia Racedo Claudine Jacquot María Serradell María C Urdaci Google Scholar Paula Carasi Silvia Racedo Claudine Jacquot María Serradell María C Urdaci PubMed Paula Carasi Silvia Racedo Claudine Jacquot María Serradell María C Urdaci Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.