Enterobacteria in the intestinal microbiocenosis of hivinfected children

Yu V Zakharova,M S Afanasiev,A C Bykov,L A Levanova,V I Ivanov,S S Afanasiev,A V Karaulov

doi:10.36233/0372-9311-2019-5-40-46

Abstract

Aim. The purpose was to assess the species composition, virulence factors and the nature of the interaction of Enterobacteria with intestinal microsymbionts in HIV-positive children.Material and methods. The composition of intestinal microbiocenosis of 89 HIV-infected children (main group) and 74 children without HIV status (comparison group) was studied. 273 strains of Enterobacteria were isolated and identified to the species. The frequency and level of expression of adhesion, invasion, toxin formation and antagonism factors were studied.Results. In HIV-positive children colonization rate of intestinal mucosa E. coli lac — was 5 times higher, E. coli hly+ 1.5 times higher than in the comparison group. The quantitative level of opportunistic enterobacteria increases with the aggravation of microecological disorders, 98% of enterobacteria forms a many-to-many association. Of these, 86.8% are neutral towards other symbionts. The frequency and level of production of adhesion, invasion and toxin formation factors did not differ in the compared groups.Conclusion. In HIV-infected children is formed enterobacteriales type microbiota. Biological properties of enterobacteria did not depend on the presence of HIV status in children.

Highlights

The frequency and level of production of adhesion, invasion and toxin formation factors did not differ in the compared groups
8. Paquin-Proulx D., Ching C., Vujkovic-Cvijin I. et al Bacteroides are associated with GALT iNK T cell function and reduction of microbial translocation in HIV-1 infection

Summary

ENTEROBACTERIA IN THE INTESTINAL MICROBIOCENOSIS OF HIVINFECTED CHILDREN

Aim. The purpose was to assess the species composition, virulence factors and the nature of the interaction of Enterobacteria with intestinal microsymbionts in HIV-positive children. The frequency and level of expression of adhesion, invasion, toxin formation and antagonism factors were studied. The frequency and level of production of adhesion, invasion and toxin formation factors did not differ in the compared groups. Что ВИЧ-ассоциированные изменения кишечного микробиоценоза оказывают влияние на характер течения инфекции и прогрессирование заболевания [1, 2, 3]. Это не позволяет оценить механизмы развития микроэкологических нарушений и транслокации бактерий в кровеносное русло у ВИЧ-инфицированных пациентов с позиций ассоциативного бактериального симбиоза. Актуальность исследования обусловлена также тем, что практически отсутствуют данные о вирулентных свойствах энтеробактерий, что затрудняет процесс коррекции микроэкологических нарушений кишечника у данной категории пациентов [9]. Цель исследования — оценка видового состава, факторов вирулентности и характера взаимодействия энтеробактерий с кишечными микросимбионтами у детей с ВИЧ-инфекцией

МАТЕРИАЛЫ И МЕТОДЫ

Findings

РЕЗУЛ ЬТАТ ЫИОБСУЖДЕНИЕ