Abstract

Amoebiasis is caused by Entamoeba histolytica and ranked second for parasitic diseases causing death after malaria. E. histolytica membrane and cytosolic proteins play important roles in the pathogenesis. Our previous study had shown several cytosolic proteins were found in the membrane fraction. Therefore, this study aimed to quantify the differential abundance of membrane and cytosolic proteins in membrane versus cytosolic fractions and analyze their predicted functions and interaction. Previous LC-ESI-MS/MS data were analyzed by PERSEUS software for the differentially abundant proteins, then they were classified into their functional annotations and the protein networks were summarized using PantherDB and STRiNG, respectively. The results showed 24 (44.4%) out of the 54 proteins that increased in abundance were membrane proteins and 30 were cytosolic proteins. Meanwhile, 45 cytosolic proteins were found to decrease in abundance. Functional analysis showed differential abundance proteins involved in the molecular function, biological process, and cellular component with 18.88%, 33.04% and, 48.07%, respectively. The STRiNG server predicted that the decreased abundance proteins had more protein–protein network interactions compared to increased abundance proteins. Overall, this study has confirmed the presence of the differentially abundant membrane and cytosolic proteins and provided the predictive functions and interactions between them.

Highlights

  • Amoebiasis or amoebic dysentery is a protozoan disease caused by Entamoeba histolytica, which is mainly found in the human colon

  • 24 of the increased abundant proteins were predicted as membrane proteins (Table 2) and there was no membrane protein from decreased abundance proteins (Table 3)

  • The results showed a few membrane and cytosolic proteins in the membrane fraction were predicted to be associated by the protein–protein interaction analysis

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Summary

Introduction

Amoebiasis or amoebic dysentery is a protozoan disease caused by Entamoeba histolytica, which is mainly found in the human colon. It may exist as a non-pathogenic commensal or penetrate the intestinal mucosa and metastasize to cause an extraintestinal infection like an amoebic liver abscess (ALA) [1]. The parasite has a simple two-stage life cycle including trophozoite and cyst stages. Infection of a human by E. histolytica begins by ingestion of the cyst, which is protected from the environment by a highly resistant chitin-containing cell wall [2]. Trophozoites penetrate the intestinal mucus layer that develops colitis in the colon during the disease-causing process. Trophozoite invasion includes the destruction of epithelial cells, lymphocytes, and polymorphonuclear cells [2]

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