Abstract
Recombinant Escherichia coli strains defective in FadA and/or FadB harboring the Pseudomonas sp. 61-3 polyhydroxyalkanoate (PHA) synthase gene ( phaC2 Ps ) were constructed, and were examined for the production of medium-chain-length (MCL) PHA from sodium decanoate and sodium dodecanoate. All the recombinant E. coli strains accumulated MCL-PHAs mainly composed of C6, C8 and C10 monomers from decanoate and those composed of C8, C10 and C12 monomers from dodecanoate. A new metabolic engineering strategy for enriching specific monomers in MCL-PHA was developed by examining the effect of co-expressing the E. coli fadD Ec , fadE Ec and/or fadL Ec genes along with the PHA synthase gene. Using these engineered E. coli strains, MCL-PHAs enriched in 3-hydroxydecanoate up to 80 mol% and 3-hydroxydodecanoate up to 48 mol% were produced from sodium decanoate and sodium dodecanoate, respectively. It was found that the amplification of the fadD Ec , fadE Ec or fadL Ec gene had different effect on the monomer composition of MCL-PHA. Among these, the amplification of the fadD Ec gene had the most significant effect on the alteration of monomer composition of MCL-PHAs.
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