Abstract
AbstractLysophosphatidylcholine (LPC) possesses excellent oil‐in‐water emulsifying properties and health benefits. The objective of this study was to produce an LPC‐enriched fraction from lysolecithin generated during enzymatic degumming of crude canola oil. Three alcohols (methanol, ethanol and isopropanol) were evaluated for their effectiveness at enriching LPC. A 3 × 3 full factorial design was employed to study the effects of two processing parameters (temperature and alcohol/lysolecithin ratio) on three responses (yield and LPC concentration of alcohol soluble fraction, and LPC recovery) with the most effective alcohol. Ethanol was found to be the best solvent to enrich LPC in lysolecithin. An ethanol soluble fraction with more than 50 % LPC was produced. Quadratic models with R2 > 0.9 were developed to describe the relationship between the processing parameters and the responses in the 3 × 3 full factorial experiment. Both ethanol soluble fraction yield and LPC recovery increased with increasing temperature and ethanol/lysolecithin ratio. LPC concentration in the ethanol soluble fraction was enhanced with decreasing temperature and ethanol/lysolecithin ratio. According to the analysis, ethanol soluble fractions with LPC concentration higher than 66 % could be obtained at temperatures of 0–40 °C and an ethanol/lysolecithin ratio of 2:1 (v/w).
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