Abstract
Leukocytes have an essential role in patient clinical trajectories and progression. Traditional methods of leukocyte enrichment have many significant limitations for current applications. It is demonstrated a novel 3D printing leukocyte sorting accumulator that combines with centrifugation to ensure label-free initial leukocyte enrichment based on cell density and size. The internal structure of leukocyte sorting accumulator (revealed here in a new design, leukocyte sorting accumulator-3, upgraded from earlier models), optimizes localization of the buffy coat fraction and the length of the period allocated for a second centrifugation step to deliver a higher recovery of buffy coats than earlier models. Established methodological parameters were evaluated for reliability by calculating leukocyte recovery rates and erythrocyte depletion rates by both pushing and pulling methods of cell displacement. Results indicate that leukocyte sorting accumulator-3 achieves a mean leukocytes recovery fraction of 96.2 ± 2.38% by the pushing method of layer displacement. By the pulling method, the leukocyte sorting accumulator-3 yield a mean leukocytes recovery fraction of 94.4 ± 0.8%. New procedures for preliminary enrichment of leukocytes from peripheral blood that avoid cellular damage, as well as avert metabolic and phase cycle intervention, are required as the first step in many modern clinical and basic research assays.
Highlights
Enumeration of leukocyte cells within peripheral blood provides valuable clinical information to physicians about the status of a patient
Because of the significant difference among individuals within normal-range hematocrit, the buffy coat was located in the middle one-third (1/3 to 2/ 3) position of the upper funnel after clockwise or anticlockwise rotate of the threaded-booster of leukocyte sorting accumulator (LSA)-3
The diameter of leukocyte sorting accumulator 3.0 (LSA-3) is larger than leukocyte sorting accumulator 2.0 (LSA-2) in the central channel so that the resistance of the cell exchange under centrifugal force becomes smaller than that of the LSA-2 design
Summary
Enumeration of leukocyte cells within peripheral blood provides valuable clinical information to physicians about the status of a patient. Leukemia and HIV can both be diagnosed and monitored by assessing leukocyte sub-populations [1]. These examples, as well as many others, have led to the development of new creative methods to produce and use the information about leukocytes to predict patient clinical trajectories and progression, as well as to guide treatment modalities on patient-to-patient basis [2]. As possibly the most clinically-relevant cell population in whole blood, the proportion of leukocyte cells in blood cells is very small [3]. The novel enrichment leukocytes methods that reduce the costs and analysis time.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
