Abstract
Liposomes are suitable carrier for bioactive components. Ethanol injection method was chosen for preparation liposomes included vitamin D3 for addition into dark chocolate [5µg/serving (10g)]. Assessment the quality of prepared liposomes were performed by measurement particle size distribution, zeta potential, loading efficiency and FTIR. Loading efficiency was 62.58 ± 1.2%. It could be concluded the chosen method for liposome preparation was suitable. In vitro release of vitamin D3 from liposome structure showed the release follow Korsmeyer-Peppas model. Fortified chocolate properties included Differential Scanning Calorimeter (DSC), color indexes, casson viscosity and sensory properties were evaluated. According to the results, there was no significant changes in all tested properties in fortified chocolate than control sample. Vitamin D3 retention during storage time (0, 15, 30, 45, 60 and 70days) were evaluated and results revealed there was better retention of vitamin D3 in fortified samples with liposomes than free vitamin D3. Observation showed addition both forms of vitamin D3 (free or liposome) had no significant impact on color indexes, rheological properties, DSC parameters as well as sensory characteristics.
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