Abstract

Radioactive surface and metabolic labeling techniques were used to study the surface glycoproteins which remain in detergent-resistant cytoskeletons of cultured human fibroblasts. A 140 kilodalton (kd), fucose-containing glycoprotein (gp) was enriched in the cytoskeletal preparations together with extracellular matrix fibronectin. The 140 kd gp resisted trypsintreatment and was present as a major surface glycoprotein also in cytoskeletons of newly attached cells which had deposited only minimal quantities of extracellular matrix. In isoelectric focusing the protein was separated into three spots. The results suggest that the 140 kd gp is a cytoskeleton-associated surface glycoprotein which may play a role in the attachment of the cytoskeleton to the growth substratum.

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