Abstract
In the present study, the performance and separation characteristics of six macroporous resins for the enrichment and purification of total ginkgo flavonoid O-glycosides (TGFs) (quercetin (I), kaempferol (II), isorhamnetin (III)) from Ginkgo Biloba extracts (EGB) are evaluated. The adsorption and desorption properties of TGFs are studied on macroporous resins, including D101, D201, AB-8, HPD400, D301, and D311. Along with the results, AB-8 resin exhibits the best adsorption and desorption capacity for these three ginkgo flavonoid O-glycosides among the six resins. Adsorption isotherms are created on AB-8 resin and fit well to the Langmuir (R2 > 0.96) and Freundlich (R2 > 0.92, 0.3 < 1/n < 0.7) models. After the treatment with gradient elution on AB-8 resin packed chromatography column, the contents of the three main ginkgo flavonoid O-glycosides (I, II, and III) increase from 8.93%, 9.88%, and 6.11% in the extracts to 30.12%, 35.21%, and 14.14%, respectively, in the product. The recoveries of compounds I, II, and III are 88.76%, 93.78%, and 60.90%, respectively. Additionally, the anti-inflammatory effects of TGFs are evaluated in LPS-treated RAW 264.7 macrophages, and the result demonstrates that TGFs could significantly inhibit LPS-induced NO release in vitro in a dose-dependent manner compared with the control group. These findings suggest that TGFs could potentially be natural antioxidants and anti-inflammatory ingredients that could be used in pharmaceutical products and functional food additives.
Highlights
Ginkgo biloba is called a living fossil and has been surviving on Earth for 180 million years, and EGB has been used for medicinal purposes in China for several thousand years
Six macroporous resins ranging macroporous resins are applicable to the adsorption of total ginkgo flavonoid O-glycosides (TGFs)
Six macroporous resins ranging from non‐polarity to for adsorption adsorption of
Summary
Ginkgo biloba is called a living fossil and has been surviving on Earth for 180 million years, and EGB has been used for medicinal purposes in China for several thousand years. HighThese adsorption capacity, efficiency, charge, effortless and simple procedure advantages makehigh macroporous cheap charge, effortless regeneration and simple procedure [6,7]. These advantages make macroporous resin useful, in the field of water treatment, and in the extraction and purification of resin useful, from in thethe field ofextracts water treatment, and in the extraction and purification of bioactive components raw of herbal plants [8,9,10]. Efficient method for the preparative of of from EGB with an on optimal resin.
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