Enriched separation of protopanaxatriol ginsenosides, malonyl ginsenosides and protopanaxadiol ginsenosides from Panax ginseng using macroporous resins

Abstract

This study developed a method for enriched separation of protopanaxatriol ginsenosides, malonyl ginsenosides and protopanaxadiol ginsenosides from Panax ginseng. The method began with targets extraction from herbal material, followed by HPD300 resins separation, then extracted by water-statured n-butanol. In the product achieved, protopanaxatriol ginsenosides were 24.4% pure with 70.9% recovery, malonyl ginsenosides were 33.4% pure with 23.3% recovery, and protopanaxadiol ginsenosides as well as Ro were 31.3% pure with 56.8% recovery. The adsorption test data on HPD300 resins fit the Langmuir model well. Moreover, the pseudo-first-order kinetic model showed the best correlation with the adsorption results. These findings indicate that the combination of HPD300 resin chromatography and water-statured n-butanol extraction effectively separates and enriches ginsenosides from P. ginseng.