Abstract
This work is directed to the estimation of the analytical parameters for improved immunochromatographic tests. A combination of 2 approaches has been proposed to decrease the detection limit of troponin T, a biomarker of acute myocardial infarction. The first approach consists of replacing spherical gold nanoparticles, which are common markers in immunochromatography, with alternative markers, such as gold nanoflowers (AuNFs). The second approach consists of the formation of large aggregates in the analytical zone from AuNFs due to the biotin–streptavidininteraction. The improved tests demonstrated a 10-fold lowering of the detection limit (1.2 ng/ml instead of 11.1 ng/ml) and a 3-fold expanding of the working range of the determined concentrations.
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