Enhancing the resolution of multi-dimensional heteronuclear NMR spectra of intrinsically disordered proteins by homonuclear broadband decoupling

N Helge Meyer,Klaus Zangger

doi:10.1039/c3cc48135b

Abstract

Limited spectral resolution in the proton dimension of NMR spectra is a severe problem in intrinsically disordered proteins. Here we show that homonuclear broadband proton decoupling of the direct and indirect dimensions of multi-dimensional NMR spectra significantly enhances their resolution.

Highlights

Limited spectral resolution in the proton dimension of NMR spectra is a severe problem in intrinsically disordered proteins
We show that homonuclear broadband proton decoupling of the direct and indirect dimensions of multi-dimensional NMR spectra significantly enhances their resolution
Limited spectral resolution is a severe problem in intrinsically disordered proteins (IDPs),[1] whose chemical shifts are often close to random coil values

Summary

Introduction

Enhancing the resolution of multi-dimensional heteronuclear NMR spectra of intrinsically disordered proteins by homonuclear broadband decoupling† Limited spectral resolution in the proton dimension of NMR spectra is a severe problem in intrinsically disordered proteins. We show that homonuclear broadband proton decoupling of the direct and indirect dimensions of multi-dimensional NMR spectra significantly enhances their resolution.

Results

Conclusion