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Abstract
Purpose: To improve the production of a novel exopolysaccharide (EPS) by Bacillus mucilaginosus CGMCC5766.Methods: The culture medium for production of EPS was optimized using statistical experiment design. Sucrose, CaCO3 and K2HPO4 were found to be the key factors based on the results obtained from Plackett–Burman experimental design. The optimal concentration range of the three factors was examined by the steepest ascent path, and then the optimum concentration was further investigated through response surface methodology. Validation experiments were also carried out to establish the adequacy and accuracy of the model obtained.Results: Under optimal conditions (sucrose 61.31 g/l, CaCO3 5.06 g/l and K2HPO4 0.17 g/l), EPS concentration achieved 22.04 g/l, which was about 1.6-fold increase compared with that using the original medium. EPS concentration in 5-l fermenter reached 23.46 ± 0.76 g/l.Conclusion: The optimization medium is considered fundamental and useful for the development of B. mucilaginosus CGMCC5766 cultivation process for efficient production of EPS on a large scale.Keywords: Bacillus mucilaginosus CGMCC5766, Exopolysaccharide, Medium, Optimization, Statistical experiment design
Highlights
Microorganism exopolysaccharides (EPSs) are biopolymers which either attach to the cell surface or are excreted in the extracellular medium in the form of amorphous slime [1]
Previous studies indicated that the factors such as Na+, Mg2+ and phosphate might have important effects on the production of EPS during the fermentation of B. mucilaginosus [9,14]
Based on the analysis by design expert, a first-order model could be obtained from the regression results of Plackett– Burman (P–B) experiment: Y=11.73+2.03X1+0.42X2+2.68X3+0.46X4-0.97X5
Summary
Microorganism exopolysaccharides (EPSs) are biopolymers which either attach to the cell surface or are excreted in the extracellular medium in the form of amorphous slime [1]. Many microorganisms have been shown to produce EPS e.g., Xanthomonas campestris [2], Pseudomonas aeruginosa [3], Sphingomonas paucimobilis [4], Streptococcus zooepidemicus [5], Haloferax mediterranei [6]. Due to their many interesting physical and chemical properties, microbial EPS are widely used in several fields such as food, coating materials, medicine, concrete additives, enhanced oil recovery and wastewater treatment as a suspending, stabilizing, emulsifying, thickening agent and flocculants [1]. It is important to reduce the costs of the medium as this may affect the overall process economics
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