Abstract

Microcystis aeruginosa, a freshwater microalga, is capable of producing and accumulating different types of sugars in its biomass which make it a good feedstock for bioethanol production. Present study aims to investigate the effect of different factors increasing growth rate and carbohydrates productivity of M. aeruginosa. MF media (modified BG11 media) and additional ingredients such as aminolevulinic acid (2 mM), lysine (2.28 mM), alanine (1 mM), and Naphthalene acetic acid (1 mM) as cytokine promoted M. aeruginosa growth and sugar contents. Salmonella showed growth-assisting effect on M. aeruginosa. Enhanced growth rate and carbohydrates contents were observed in M. aeruginosa culture grown at 25°C under red LED light of 90 μmolm−2s−1 intensity. More greenish and carbohydrates rich M. aeruginosa biomass was prepared (final OD660 nm = 2.21 and sugar contents 10.39 mM/mL) as compared to control (maximum OD660 nm = 1.4 and sugar contents 3 mM/mL). The final algae biomass was converted to algae juice through a specific pretreatment method. The resulted algae Juice was used as a substrate in fermentation process. Highest yield of bioethanol (50 mM/mL) was detected when Brettanomyces custersainus, Saccharomyces cerevisiae, and Pichia stipitis were used in combinations for fermentation process as compared to their individual fermentation. The results indicated the influence of different factors on the growth rate and carbohydrates productivity of M. aeruginosa and its feasibility as a feedstock for fermentative ethanol production.

Highlights

  • The depletion of fossil fuels due to continuous consumption throughout the world and their contribution in environmental pollution and global warming shifted the interests of researchers to explore sustainable, economical, and ecofriendly energy sources alternative to petroleum based fuels [1, 2]

  • The effect of a parameter on algal growth was determined by the increase in biomass and sugar content of M. aeruginosa culture

  • The growth rate of M. aeruginosa was increased in the modified medium compared to that in the original BG11 medium (Figure 1)

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Summary

Introduction

The depletion of fossil fuels due to continuous consumption throughout the world and their contribution in environmental pollution and global warming shifted the interests of researchers to explore sustainable, economical, and ecofriendly energy sources alternative to petroleum based fuels [1, 2]. Despite the adventitious aspects of algae as a potential feedstock for bioethanol production, there had not been done an appreciable work in this field The research in this area is limited [12, 13]. The algal carbohydrates are of great importance for bioethanol production and some algae species possess more carbohydrates contents than lipids. The amounts of sugars in the algal biomass are the basic and major requirements for bioethanol production. Higher yield of bioethanol needs algal biomass with higher amounts of carbohydrates. Our aim of this work was to investigate the optimum conditions and nutrient composition for growing M. aeruginosa with higher growth rate and carbohydrates contents in order to obtain a carbohydrates rich algal biomass for generating higher yield of bioethanol. The difference in growth pattern was monitored and the morphology of UV-treated algae was investigated with an image analyzing system (Nikon, Japan)

Materials and Methods
Results and Discussions
Cytokine
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