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Abstract
Polysaccharopeptides (PSPs) are among the main bioactive constituents of Trametes versicolor (T. versicolor). The purpose of this research was to investigate the antioxidant activities of enzymatic hydrolysates obtained from T. versicolor polysaccharopeptides by 80 U/mL β-1,3-glucanase (PSPs-EH80). The half-inhibitory concentration (IC50) of PSPs-EH80 in metal chelating assay, ABTS and DPPH radical scavenging test results were 0.83 mg/mL, 0.14 mg/mL and 0.52 mg/mL, respectively, which were lower than that of PSPs-EH 20 U/mL. The molecular weights of the PSPs-EH80 hydrolysates were 300, 190, 140 and 50 kDa, respectively, and the hydrolysis of polysaccharides by β-1,3-glucanase did not change the original functional group. PSPs-EH80 reduced the reactive oxygen species (ROS) content at least twice that of treatment without PSPs-EH80. In addition, an oxidative damage test showed that PSPs-EH80 can improve HaCaT cell survival. According to our results, PSP demonstrates the potential of anti-oxidative damage; besides, enzyme hydrolysis can improve the ability of the PSP.
Highlights
Trametes versicolor (T. versicolor) is a basidiomycete that has been used as a traditional medicinal mushroom [1]
The results revealed that β-1,3-glucanase can hydrolyze the macromolecules of PSPs into smaller molecules, and that the hydrolysis rate was 38.6% (Table 1)
Our results suggest that PSP-EH80 could prevent cell death induced by azobis(2-amidinopropane) dihydrochloride (AAPH) through inhibiting
Summary
Trametes versicolor (T. versicolor) is a basidiomycete that has been used as a traditional medicinal mushroom [1]. Enzymatic hydrolysis can cleave the glycosidic bonds in a necessary bioactive groups. Enzymatic hydrolysis can cleave the glycosidic bonds in polysaccharide chain [10,14]. Glucanases can be divided into exo- and endo- depending on the different positions of the glucan bonds they hydrolyze [15]. Β-1,3-Glucanase, an endoglucanase different positions of the glucan bonds they hydrolyze [15]. To hydrolyze β-(1→3)-glycosidic linkages in PSPs, β-(1→3)-D-glucanase was used to obtain enzymatic hydrolysates from T. versicolor PSPs. The antioxidant activities and was used to obtain enzymatic hydrolysates from T. versicolor PSPs. The antioxidant activities and functional groups of different molecular weight enzymatic hydrolysates were analyzed. The use of functional groups of different molecular weight enzymatic hydrolysates were analyzed.
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