Enhancing stability and antioxidant efficacy of fisetin by encapsulating as β-cyclodextrin inclusion complex with porous polylactic acid film from breath figure

Abstract

This research aims to investigate the scope of fisetin (FIT)/β-cyclodextrin (β-CD) inclusion complexes with the ratios of 1:2 and 2:1 mole by means of freeze-drying, kneading and physical mixing methods (controlled process). Moreover, the complex compounds are analyzed by Fourier-Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC). The results show that the most suitable preparation method of fisetin (FIT)/β-CD complex is the freeze-drying method with the fisetin (FIT) to β-CD ratio at 1:2. The emission of FIT/β-CD was investigated and it is found that the emission rate of freeze-dried FIT/β-CD in 1:2 ratio by freeze-drying method is lower than that by kneading and physical mixing methods. These results lead to an initiation of the innovative active packaging materials with synthetic polylactic acid (PLA) porous film by breath figure (BF) method in order to entrap FIT/β-CD inclusion complex. The honeycomb structure with and without the FIT/β-CD complex were analyzed by Scanning Electron Microscopy (SEM). Thereafter, the effectiveness of Antioxidant Activities (%AA) of the porous PLA films is measured by the anti-oxidation caused by 2,2-diphenyl-1-picrylhydrazyl (DPPH method). The result shows that PLA entrapping complex fisetin is more effective than PLA entrapping pure fisetin, by result of 53.0% and 48.6%, respectively.