Enhancing quinoa (Chenopodium quinoa) growth in saline environments through salt-tolerant rhizobacteria from halophyte biotope.

Abstract

The use of plant growth-promoting rhizobacteria (PGPR) in agriculture is one of the most promising approaches to improve plants' growth under salt stress and to support sustainable agriculture under climate change. In this context, our goal was to grow and enhance quinoa growth using native rhizobacteria that can withstand salt stress. To achieve this objective, we isolated rhizobacteria from three saline localities in a semi-arid region in Tunisia, which are characterized by different halophyte species and tested their plant growth-promoting (PGP) activities. Then, we inoculated quinoa seedlings cultivated on 300 mM NaCl with the three most efficient rhizobacteria. A positive effect of the three-salt tolerant rhizobacteria on the growth of quinoa under salinity was observed. In fact, the results of principal component analysis indicated that the inoculation of quinoa by salt-tolerant PGPR under high salinity had a prominent beneficial effect on various growth and physiological parameters of stressed plant, such as the biomass production, the roots length, the secondary roots number, proline content and photosynthesis activities. Three rhizobacteria were utilized in this investigation, and the molecular identification revealed that strain 1 is related to the Bacillus inaquosorum species, strain 2 to Bacillus thuringiensis species and strain 3 to Bacillus proteolyticus species. We can conclude that the saline soil, especially the halophytic rhizosphere, is a potential source of salt-tolerant plant growth-promoting rhizobacteria (ST-PGPR), which stimulate the growth of quinoa and improve its tolerance to salinity.