Enhancing propagation efficiency of banana cv. MONTHAN (ABB) through micropropagation

Abstract

An investigation was carried out at Plant Tissue Culture Laboratory of Horticultural College and Research Institute, Coimbatore during 2012-2013. Shoot tip of banana cv. MONTHAN were cultured in MS medium fortified with different growth regulators (BAP @ 4, 5 and 6 mg l -1 ) either alone or in combination with NAA (0.5 mg l -1 ) and kinetin (2 mg l -1 ) to study the influence of growth regulators on multiplication rate. The data on number of multiple shoots, days for multiple shoot induction and micro shoot length were recorded. MS+BAP 6 mg l -1 + kinetin 2 mg l -1 recorded maximum number of multiple shoots (5.07). MS + BAP 6 mg l -1 recorded least days for multiple shoot induction (27.06 days) and highest micro shoot length (6.73cm). Further to enhance multiplication best growth regulator combination (MS + BAP 6 mg l -1 + Kinetin 2 mg l -1 ) along with different concentration of coconut water (5, 10 and 15%) and casein hydrolysate (100, 200 and 300 mg l -1 ) were supplemented. MS + BAP 6 mg l -1 + kinetin 2 mg l -1 +coconut water 5 per cent recorded the highest number of multiple shoots at all the intervals of subculture (5.13). The best growth regulator combination (MS+BAP 6 mg l -1 + kinetin 2 mg l -1 ) along with B5 vitamins in specific to different thiamine concentration (5, 10 and 15 mg l -1 ) for further multiplication were supplemented and B 5 vitamins with thiamine combinations did not increase the multiplication rate. The degree of efficiency of shooting was found to be dependent on type of hormone.