Abstract
Alkaline polygalacturonate lyase (PGL, EC 4.2.2.2) is an enzyme used in many industries. We developed a fed-batch fermentation process that combines the enzymatic pretreatment of the carbon source with controlling the pH of the fermentative broth to enhance the PGL production from Bacillus subtilis 7-3-3 to decrease the production cost. Maintaining the fermentation broth at pH 6.5 prior to feeding with ammonia and at pH 6.0 after feeding significantly improved PGL activity (743.5 U mL−1) compared with the control (202.5 U mL−1). The average PGL productivity reached 19.6 U mL−1 h−1 after 38 h of fermentation. The crude PGL was suitable for environmentally friendly ramie enzymatic degumming.
Highlights
Ramie (Boehmeria nivea) fibers, the longest, strongest, and silkiest among plant fibers, are widely used in the textile industry because of their excellent properties, such as preeminent absorption, shrinkage resistance, easy dyeing, and quick drying, as well as their good mildew, bacteria, and insect resistance [1]
The gum (20% to 30%) in ramie fibers, which consists of pectin and hemicellulose, must first be removed before they can be applied to the textile industry [2].Large amounts of chemicals and energy are consumed during conventional chemical degumming, causing serious environmental pollution
The main enzymes used in the process are alkaline pectinases, among which alkaline polygalacturonate lyase (PGL) is the main component [2,4]
Summary
Ramie (Boehmeria nivea) fibers, the longest, strongest, and silkiest among plant fibers, are widely used in the textile industry because of their excellent properties, such as preeminent absorption, shrinkage resistance, easy dyeing, and quick drying, as well as their good mildew, bacteria, and insect resistance [1]. The gum (20% to 30%) in ramie fibers, which consists of pectin and hemicellulose, must first be removed before they can be applied to the textile industry [2].Large amounts of chemicals (such as NaOH) and energy are consumed during conventional chemical degumming, causing serious environmental pollution. The main enzymes used in the process are alkaline pectinases, among which alkaline polygalacturonate lyase (PGL) is the main component [2,4]. Improving PGL activity in crude enzyme promotes its application in degumming and makes it a viable option for more biochemical processes
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