Abstract

The relationship of antigenic determinants on HLA-Bw4-bearing molecules has been analyzed by means of the monoclonal antibodies (MOABs) TU48 (IgM), TU109 (IgG) and TU110 (IgG). TU48 [1] and TU109 [C. Muller et al., in preparation] recognize HLA-Bw4 molecules, but TU48 also reacts with certain HLA-A antigens. Inhibition studies carried out with the human monocytoid cell line U937 (HLA-A3, Awl9, B18, Bw53, Bw4, Bw6, Cw1, Cw3) and the B cell line BJAB-B95.8.6 (HLA- Al, A2, B13, B35, Bw4, Bw6, Cw4) showed that TU48 and TU109 recognize two different epitopes on molecules bearing the “supertypic” specificity Bw4; neither MOAB was able to compete for binding with the other (max. level of inhibition ~ 15%). Although both MOABs react very reliably in serological assays, they are problematic reagents for immunoprecipitations. The MOAB TU48 does not precipitate its antigen from 125I-labeled lysates of Bw4/Bw6 or Bw4/Bw4 cells.

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