Enhancing microbial community performance on acid resistance by modified adaptive laboratory evolution

Abstract

A new strategy of three-step adaptive laboratory evolution (ALE) was developed to enhance the bioleaching performance of moderately thermophilic consortia. Through consortium construction, directed evolution and chemostat selection, an improved consortium (ALEend) that composed of Leptospirillum ferriphilum (80.32%), Sulfobacillus thermosulfidooxidans (15.82%) and Ferroplasma thermophilum (3.86%) was obtained, showing ferrous iron oxidation rate of 500 mgL−1h−1 and biomass production of 2.0 × 108 cells/mL at pH 0.75. During batch culturing, the ALEend consortium exhibited stable ferrous iron oxidation in wider conditions. PCA indicated that the communities were similar under fluctuating culture conditions, which demonstrated the stable community structure and the reinforced synergistic interactions resulting in the enhanced community performance. Pyrite bioleaching conducted at pH 1.5 and 0.75 revealed that the ALEend consortium extracted 26% and 55% more total iron relative to the original consortium. These findings indicated that the modified ALE may be a promising strategy for microbial community modification to enhance bioleaching.