Abstract

A thermotolerant fermentative mixed consortium (TFMC) has been screened by directional high temperature stress acclimation for biohydrogen production. The kinetics and metabolic characteristics of TFMC for hydrogen production at high temperature has also been studied. the TFMC can achieve stable hydrogen production under mesophilic conditions at 38 °C with the maximum potential hydrogen production of 665.09 mL, which is decreases by 9.27% as compared to the original mixed consortium at 30 °C. The TFMC also has characteristic of short lag time of 27 h which is 22 h lower than initial group and high hydrogen production rate of 9.48 mL/h which improved 12.9%. The newly obtained TFMC highlights a great potential for biohydrogen production in tropical areas.

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