Abstract
The biomass yield of Arthrospira mutant ZJU9000 was 176% higher than that of wild type on day 4, and the results of transcriptome sequencing showed that processes related to cell growth were synergistically enhanced in this mutant. The amount of energy for biomass accumulation increased because the efficiency of the photoreaction was enhanced by the elevated levels of chlorophyll a and carotene. The increased biosynthesis rates of ribose phosphate, nucleotides and multiple vitamins increased the production of genetic materials for cell proliferation. Furthermore, the carbon concentration mechanism in mutant ZJU9000 was enhanced, indicating the increased utilization efficiency of CO2 at low concentration (0.04 vol% in air). The enhancement of these growth-relevant metabolic pathways contributed to the robust growth of Arthrospira mutant ZJU9000.
Highlights
Phytoplanktons, including cyanobacteria as a major group, are responsible for approximately 50% of photosynthesis worldwide, thereby dominating the global conversion of CO2.1 Arthrospira (Spirulina) is one of the fastest growing cyanobacteria
The biomass yield of ZJU9000 was 176% higher than the wild type on the 4th day, and the rate of cell proliferation in the former increased, thereby increasing the amount of substrates of genetic materials, such as nucleotides and ribose 5-phosphate
High-throughput sequencing was used for the systematic analysis of pathway expression levels in wild type Arthrospira and ZJU9000
Summary
Phytoplanktons, including cyanobacteria as a major group, are responsible for approximately 50% of photosynthesis worldwide, thereby dominating the global conversion of CO2.1 Arthrospira (Spirulina) is one of the fastest growing cyanobacteria. Improving Arthrospira's growth rate increases the amount of CO2 it can assimilate and alleviates the greenhouse effect. Its biomass can be used as food and feed supplements.[4,5] Performing genetic analysis on Arthrospira can help us further understand the mechanisms underlying different growth phenotypes between strains. Two resources allowed us to investigate different Arthrospira phenotypes at the genetic level. PCC9438 became available,[6] and effective gene identi cation through high-throughput sequencing technology has become feasible.[7] Secondly, the Kyoto Encyclopedia of Genes and Genomes (KEGG) resource (http://www.genome.jp/kegg/) provided a reference knowledge database for linking genomes to biological systems and the wiring diagrams of interaction networks and reaction networks (KEGG pathway). The continuous increase in the amount of genomic and molecular
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