Abstract
In order to silence the expression levels of pathogenic genes, small interfering RNA (siRNA) requires a nano-sized carrier for its safe and stable delivery into cells. In this research highlight, we focus on well-defined cationic nanohydrogel particles developed in our group for such purposes. To investigate the nanogels’ mechanism for enhanced knockdown efficiencies, we recently synthesized two sets of particles with similar material composition and siRNA-loading characteristics, but – according to the manufacturing process – of different sizes. Within this study, 100-nm-sized nanogel particles loaded with siRNA accumulated inside the lysosomes already after 4 h and could not induce any gene knockdown, while 40-nm-sized particles were able to avoid lysosomal accumulation, and instead, generated moderate gene knockdown levels lasting for about three days. We believe that in analogy to other reports this size-dependent intracellular distribution behavior might be an essential key parameter for tuning the knockdown efficiency of the nanogel carriers. Moreover, these results might further contribute to the development of advanced siRNA carrier systems in order to enhance RNAi’s translation into the clinics.
Highlights
Already very early after its discovery RNA intereference (RNAi) [1, 2] was considered as promising therapeutic alternative for the treatment of various pathogenic genes [3, 4]
100-nm-sized nanogel particles loaded with small interfering RNA (siRNA) accumulated inside the lysosomes already after 4 h and could not induce any gene knockdown, while 40-nm-sized particles were able to avoid lysosomal accumulation, and instead, generated moderate gene knockdown levels lasting for about three days
We believe that in analogy to other reports this size-dependent intracellular distribution behavior might be an essential key parameter for tuning the knockdown efficiency of the nanogel carriers. These results might further contribute to the development of advanced siRNA carrier systems in order to enhance RNAi’s translation into the clinics
Summary
Already very early after its discovery RNA intereference (RNAi) [1, 2] was considered as promising therapeutic alternative for the treatment of various pathogenic genes [3, 4]. In order to silence the expression levels of pathogenic genes, small interfering RNA (siRNA) requires a nano-sized carrier for its safe and stable delivery into cells. To investigate the nanogels’ mechanism for enhanced knockdown efficiencies, we recently synthesized two sets of particles with similar material composition and siRNA-loading characteristics, but – according to the manufacturing process – of different sizes.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
