Abstract
Using the Abbott PLEX-ID PCR/mass spectrometry-based STR assay covering a total of 13 CODIS loci, we typed 310 blood samples from unrelated Russian individuals all had full STR profiles generated with the conventional ABI Identifier Plus/CE system. The MS platform provides for determination of PCR product base compositions thus enabling to determine when an allele has a SNP within the amplified region. Sequence-based polymorphisms relative to the reference alleles were seen in 10 of the 13 loci tested. Up to 15 variant alleles in particular locus were observed. At the 8 loci at least one representative heterozygous individual was found for which the locus is called homozygous by conventional typing. The results demonstrate potential advantages of the sequence-based polymorphisms assay in resolution of samples when limited number of loci can be STR-typed.
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More From: Forensic Science International: Genetics Supplement Series
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