Enhancing enzymatic hydrolysis efficiency of crop straws via tetrahydrofuran/water co-solvent pretreatment

Abstract

Pretreatment and enzymatic hydrolysis are critical steps in bio-ethanol production from lignocellulose. The enhancement of enzymatic hydrolysis of several typical crop straws and the particle size adaptability of sorghum straw by tetrahydrofuran/water co-solvent pretreatment were studied. Efficient cellulose conversions (>83.2%) and adequate hemicellulose conversion (>40.7%) were obtained from pretreated rice straw, sorghum straw, wheat straw and corn stover in enzymatic hydrolysis, and the highest glucose yield and xylose yield were 274.0 and 26.3 mg/g dry solid, respectively. Glucose production of 140.4 mg/mL was obtained when the pretreated sorghum straw with mixed particle sizes was employed in enzymatic hydrolysis at 20% solid loading (8 FPU/g cellulose). When the enzyme loading reduced to 4 FPU/g cellulose, 221.7 mg/g dry solid glucose yield and 68.6% enzymatic hydrolysis efficiency could be still obtained with 15% solid loading, exhibiting high potential for bio-ethanol production.