Enhancing direct interspecies electron transfer in syntrophic-methanogenic associations with (semi)conductive iron oxides: Effects and mechanisms

Abstract

Anaerobic digestion is an effective biological treatment process that produces methane by degrading organic compounds in waste/wastewater. It is a complicated microbial process by metabolic interactions among different types of microorganisms. In this process, efficient interspecies electron transfer between secondary fermenting bacteria and methanogens is the critical process for fast and effective methanogenesis. In syntrophic metabolism, hydrogen or formate has been considered as the conventional electron carrier transferring electrons from secondary fermenting bacteria to hydrogenotrophic methanogens. Recently, direct interspecies electron transfer (DIET) without the involvement of dissolved redox mediators is arousing great concerns and has been regarded as a more efficient and thermodynamically favorable interspecies electron transfer pathway for methanogenesis. Interspecies electron exchange through DIET is accomplished via the membrane-bound cytochromes or conductive pili. Several kinds of exogenously-added conductive or semiconductive iron oxides have been discovered to greatly enhance anaerobic methanogenesis through promoting DIET. Different (semi)conductive iron oxides give a boost to DIET through different mechanisms based on the physicochemical properties of the iron oxides and the reciprocal interactions between iron oxides and functional microorganisms. In this review, the current understanding of interspecies electron transfer in syntrophic-methanogenic consortions is summarized, the effects and deep-rooted mechanisms of (semi)conductive iron oxides on methanogenesis and DIET are discussed, and possible future perspectives and development directions are suggested for DIET via (semi)conductive iron oxides in anaerobic digestion.