Enhancing degradability, bioactivity, and osteocompatibility of poly (propylene fumarate) bone filler by incorporation of Mg-Ca-P nanoparticles.

Abstract

As an alternative for polymethyl methacrylate, poly(propylene fumarate) (PPF) has been considered as injectable and biodegradable bone cement; however, its mechanical and biological properties need more attention. Hence, the current study aimed to develop the properties by compositing PPF with magnesium calcium phosphate (MCP) nano-powders. In this regard, the pure PPF was compared with PPF/MCP by evaluating their composition, mechanical properties, hydrophilicity, and biodegradability. Furthermore, their bioactivity in the simulated body fluid (SBF) and, via applying MG-63 cells, their cell interaction, including proliferation, adhesion, differentiation, and mineralization, were assessed. The addition of MCP improved compressive strength and elastic modulus of PPF, e.g., 10wt% MCP increased them to 32.7 and 403MPa, respectively. Also, hydrophilicity and biodegradation of PPF were enhanced in the presence of MCP; so that the highest hydrophilicity, 42% higher than PPF, was achieved at the presence of 20wt% MCP. In this condition, after 21-day immersion in SBF, the surface of the sample was covered with a dense and continuous layer of hydroxyapatite. The composite proliferation, adhesion, differentiation, and mineralization of MG-63 cells improved in comparison to the pure PPF. Hence, controllable strength and biodegradation of the composite, along with its proved bioactivity and osteoconductivity, make PPF/MCP as a candidate for bone therapeutic application.