Enhancing crotonaldehyde biodegradation in petrochemical wastewater: Role of electron donors in microbial electron transfer systems

Abstract

Crotonaldehyde is typical inhibitory compound of wastewater discharged in the petrochemical industry. To resolve the inhibition of crotonaldehyde on microorganism, electron donors (cellose, glucose and ethanol) were dosed to accelerate the biodegradation of crotonaldehyde by semi-continuous operation experiments. The results indicated that crotonyl alcohol was the most dominant biodegradable product of crotonaldehyde, and its conversion rates dosing ethanol, glucose and cellose as electron donors (2000 mgCOD/L) were 1.84, 1.46 and 1.21 times higher than that in control test when the initial concentrations of crotonaldehyde was 500 mg/L, respectively. Furthermore, the ethanol as electron donor was superior to cellose and glucose in relieving the inhibition of crotonaldehyde on the processes of acidogenesis, acetogenesis, and methanogenesis duo to the more electron release, faster electron transfer efficiency, less electron competition. Last, the enrichment of electro-active bacteria such as Syntrophobacter, Geobacter and the bacteria associated with reverse β-oxidation pathway (RBO) such as Clostridium_sensu_stricto_12 also proved that higher electron transfer system (ETS) activity was obtained in the reactor dosed ethanol as electron donor. This study suggests that dosing electron donors is an efficient strategy to accelerate the biodegradation of crotonaldehyde, and the results could be referred to pretreat wastewater containing aldehyde pollutants.