Abstract

Enhancing lipid productivity and reducing oxidative damage is essential for lipid overproduction in microalgae. In this study, addition of 20 mg/L fulvic acid (FA) resulted a 34.4% increase of lipid yield in Schizochytrium sp. Furthermore, the cooperative effect of FA and EDTA on cell growth and lipid production was investigated. The combined addition of 20 mg/L FA and 1.0 g/L EDTA yielded a maximal cell dry weight of 130.7 g/L and lipid productivity of 1.16 g/L/h, representing 36.4% and threefold increase over the non-supplemented group, respectively. Moreover, compared with the non-supplemented group, the combined addition strategy exhibited overall lower levels of reactive oxygen species and malondialdehyde, which accompanied with 66.7% and 81.9% higher superoxide dismutase and catalase activity, respectively. Furthermore, a 24.1–37.1% increase of malic enzyme and 19.4–25.2% decrease of phosphoenolpyruvate carboxylase activity was observed during the entire fermentation stage (0–108 h). Results suggested that the combined addition strategy not only enhanced lipid accumulation, but also prevented the lipid peroxidation.

Highlights

  • Microalgae have received growing interest as a potential biofuel feedstock, and are regarded as a promising alternative source for next-generation renewable fuels (Bellou et al 2014)

  • Microalgal lipids are classified into two types according to their carbon number, with fatty acids containing 14–20 carbons used for biodiesel production, and polyunsaturated fatty acids (PUFA) with more than 20 carbon atoms used as health food supplements

  • Effect of fulvic acid (FA) on biomass and lipid production Addition of FA at various concentrations (0, 10, 15, 20, 25 and 30 mg/L) into the medium would result in markedly changes in the cell growth and lipid accumulation of Schizochytrium sp

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Summary

Introduction

Microalgae have received growing interest as a potential biofuel feedstock, and are regarded as a promising alternative source for next-generation renewable fuels (Bellou et al 2014). Can accumulate over 50% of lipids with a high fraction of PUFA in its cell dry weight (Ling et al 2015; Zhao et al 2018). It received increasing attention as a promising feedstock for the sustainable production of biofuels and food (Markou and Nerantzis 2013). Previous studies mainly focus on improving the fermentation process to enhance the lipid content of Schizochytrium sp., and the stress-based strategy is considered as the most successful inducing method (Sahin et al 2018). The general countermeasure is to adopt the two-stage culture strategy, apply the optimal growth conditions at the first stage into maximizing the biomass production, and preserve the lipid accumulation at the second stage.

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