Enhancing Astragaloside Production in Astragalus membranaceus through Growth Media and Auxins

Yun Ji Park,Sang Un Park,Jae Kwang Kim

doi:10.3844/ojbsci.2018.116.122

Abstract

The secondary metabolites of triterpenoid saponins, isoflavonoids and polysaccharides are present in Astragalus membranaceus those are biologically active. Astragalosides are a major group of identified compounds that have various pharmacological properties. The present study was considered to enhance astragaloside biosynthesis in hairy root cultures of A. membranaceus by using different growth media and concentrations of different auxins. Our findings revealed that Schenk and Hildebrandt (SH) medium responded greatly for the production of the highest dry weight. However, the highest astragaloside production was detected while using SH medium of its half strength. The growth of hairy roots and accumulation of astragaloside in response to different auxinlike Indole-3-Acetic Acid (IAA), Indole-3-Butyric Acid (IBA) and 1-naphthaleneacetic acid (NAA) were different. Hairy root growth rate was increased by the treatment of IBA and NAA, but was unaffected by IAA. Accumulation of Astragaloside was enhanced in response to the treatment of IBA and NAA. In most cases, treatment with the 0.5 mg L-1 NAA accumulated the highest level of stragaloside in the hairy root of A. membranaceus, which might, therefore, be utilized as an ideal mass-scale production system for astragalosides.

Highlights

Tissue cultures are generating increasing interesting as potential alternatives for achieving useful and valuable biologically active secondary metabolites (Murthy et al, 2014)
The level of astragalosides was significantly responded by the media type and salt strength (Fig. 4)
The levels of astragalosides were higher in the half-strength media than they were in the full-strength media

Summary

Introduction

Tissue cultures are generating increasing interesting as potential alternatives for achieving useful and valuable biologically active secondary metabolites (Murthy et al, 2014). Dried root of Astragalus membranaceus, very usually known as Huang Qi, is broadly used in traditional Chinese medicine (Kwon et al, 2013). Several biologically active constituents are present in A. membranaceus roots, such as saponins, isoflavonoids, polysaccharides and astragalosides. Astragalosides belong to the class of cycloartane are principal constituents that are used as quality control markers of the root (Fig. 1) (Kwon et al, 2013). The astragaloside IV has the potentiality to use as a neuroprotective, anti-inflammatory and cardio protective agent and has effects on osteogenesis, angiogenesis and metabolic syndrome (Bian et al, 2011; Luo et al, 2004; Xu et al, 2008; Zhang et al, 2011a; 2011b; 2005)

Methods

Results

Discussion

Conclusion