Abstract
Objective To explore the antitumor effect of cisplatin combined with low-frequency ultrasound on human esophageal squamous cell carcinoma in vitro.Methods Four modes of treatment were used:exposure to low-power ultrasound,application of cisplatin,exposure to ultrasound followed by cispla-tin,and presence of cisplatin followed by exposure to application ultrasound.Ultrasound was used at inten-sities of 0.5 W/cm2 and 1.0 W/cm2 for 10 min,and cisplatin was applied at concentration of 1 μ mol/L per cell suspension treated in EC9706 cells,respectively.The results of each experimental treatment were assessed by cell counting kit-8 (CCK-8) assay.Flow cytometry was performed to examine the apoptosis rate of cells treated with Annexin V-fluoresceine isothiocyanate (FITC)/proliferation index (PI) double staining.Results The combined effect of ultrasound and cisplatin was more effective than that of ultrasound or cisplatin alone.It also appeared that the order of application played a role,with the cisplatin-ul-trasound treatment lowering cell viability more than the ultrasound-cisplatin treatment.Conclusion Low-frequency ultrasound can promote the cytotoxicity of cisplatin and the order of application played a role in enhancing the sensitivity of chemotherapy drugs on tumor cells. Key words: Low-frequency ultrasound; Cisplatin; Esophageal squamous cell carcinoma; Apoptosis
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