Enhancing antibacterial action of gauze by adding gelatin nanoparticles loaded with spectinomycin and chloramphenicol

Abstract

Proteins are biocompatible, metabolizable, and susceptible to surface changes and legend attachments for targeted distribution, and are therefore ideal materials for nanoparticle-based drug delivery applications. The production, characterization, and use of gelatin nanoparticles (GNPs) for intracellular administration of weakly cell-penetrating antibiotics (such as spectinomycin and chloramphenicol) to enhance their treatment of bacterial and fungal infections are described in this paper. Gelatin nanoparticles were synthesized using the desolvation method and then loaded with two antibiotics (spectinomycin and chloramphenicol) for addition to cellulosic cotton medical gauze. The concentration of gelatin and a crosslinker were chosen and analyzed among many factors to maximize the particle size of the nanoparticles. Fourier transform infrared spectroscopy, particle size analyzers, and antibacterial activity determination were used to evaluate the medical gauze treated with the nanoparticles that were loaded with antibiotics. The results revealed that gelatin nanoparticles loaded with the antibiotics and the treated cellulosic cotton gauze exhibit higher antimicrobial activity (than the non-loaded particles and untreated gauze) against the bacteria and fungi. This resulted from the presence of antibiotics and the safety of the nanostructure as its biocompatibility with skin cells.